Differentiation media 2 (DM2)
| Reagent | Stock concentration | Final concentration | Volume |
|---|---|---|---|
| CM | 50 mL | ||
| Insulin | 2 mg/mL | 1 μg/mL | 25.0 μL |
| Total | N/A | N/A | ∼50.0 mL |
Note: DM2 must sterile filtered (0.2 μm filter) before usage and can be stored up to two weeks.
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