REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
1x D-PBS	Thermo Fisher Scientific	14190144
Dexamethasone	Sigma-Aldrich	D4902
Dulbecco’s modified Eagle’s medium (DMEM)	Thermo Fisher Scientific	41966029
Fetal bovine serum (FBS)	Thermo Fisher Scientific	10270106
Formaldehyde solution	Carl Roth	4979.2
Insulin from bovine pancreas	Sigma-Aldrich	I6634
IBMX	Sigma-Aldrich	I5879
Isopropanol	Carl Roth	9265.2
Oil red O	Sigma-Aldrich	O-0625
Penicillin-Streptomycin (10,000 U/mL) (Pen/Strep)	Thermo Fisher Scientific	15140122
Rosiglitazone	Sigma-Aldrich	R2408
Trypsin-EDTA (0.25%), phenol red	Thermo Fisher Scientific	25200056
Experimental models: Cell lines
3T3-L1 cells	ATCC	CL-173; RRID:CVCL_0123
Oligonucleotides
Non coding (siNC), rCrGrUrUrArArUrCrGrCrGrUr ArUrArArUrArCrGrCrGrUAT	OriGene	SR30004
siFzd5, rGrCrArCrUrArArGrArCrGrGrArCrArArGr CrUrArGrArGAA	OriGene	SR417789, siRNA C
Software and algorithms
BioRender	BioRender	https://biorender.com/
GraphPad Prism	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
ImageJ 1.53q	Fiji	https://fiji.sc/
Other
175 cm2 cell culture flask	Greiner Bio-One	658175
6-well plate	Greiner Bio-One	657160
12-well plate	Greiner Bio-One	665180
24-well plate	TPP Techno Plastic Products AG	92024
48-well plate	Greiner Bio-One	655180
96-well plate	Greiner Bio-One	677165
50 mL tubes	SARSTEDT GmbH	62547254
Filtropur S 0.2	SARSTEDT GmbH	83.1826.001
CO2 incubator for cell culture	Thermo Fisher Scientific	Heraeus Kendro HeraCell
Centrifuge	Thermo Fisher Scientific	Megafuge 16R
Improved Neubauer counting chamber	Th. Geyer GmbH	6261165
Compact fluorescence microscope BZ-X800	Keyence	Keyence bz-x800_long
Multimode multilabel plate reader to measure absorbance at 500 nm and 620 nm	PerkinElmer	EnVision 2104 multilabel plate reader with monochromator
Safety cabinets	Thermo Fisher Scientific	HERAsafe KS 12