Fig. 3.

G6PD modulates epileptic seizure activity. (a) Immunofluorescence labeling of enhanced green fluorescent proteins in the hippocampal CA1 region transfected with AAV-eGFP-adG6PD (n = 1). (b–c) Immunoblotting images (b) and quantification (c) of G6PD protein levels in the hippocampus of mice infected with the indicated AAVs (n = 6). Data are presented as the means ± SEM, ***P < 0.001, unpaired two-tailed Student's t-test. (d, e) Representative baseline and chronic epileptic electroencephalogram recordings from the cortex of chronic epileptic (adCtrl group) and G6PD overexpression after chronic epilepsy modeling (adG6PD group). (f, g) Quantitative analysis of the daily number of spontaneous recurrent seizures and duration of seizure-like events in the KA-induced epilepsy model in the adCtrl (n = 11) and adG6PD (n = 8) groups. Data are presented as means ± SEM. **P < 0.01, ***P < 0.001, unpaired two-tailed Student's t-test. (h–j) Representative traces and a summary of the amplitudes and frequencies of spontaneous excitatory postsynaptic currents in the hippocampal CA1 region in the adCtrl (n = 3, 6 cells) and adG6PD (n = 3, 8 cells) groups. (k–m) Representative traces and summary of the amplitude and frequency of spontaneous inhibitory postsynaptic currents in the hippocampal CA1 region of the adCtrl (n = 3, 8 cells) and adG6PD (n = 3, 8 cells) groups. Data are presented as means ± SEM.**P < 0.01, ***P < 0.001, unpaired two-tailed Student's t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)