Fig. 5.

G6PD promotes the expression of STAT1 by decreasing reactive oxygen species (ROS) (a–d) Quantification of ROS, NADPH/NADP⁺, and GSH/GSSH in the control, AG1, AG1+DMF, 6-An, and 6-An + NAC groups after kainic acid (KA) treatment of HT22 cells. Data are presented as means ± SEM. ****P < 0.0001, one-way ANOVA with Tukey's post-hoc test. (e–h) Quantification of ROS, NADPH/NADP⁺, and GSH/GSSH in the control, 6-An, 6-An, 6-An, and 6-An + NAC groups after kainic acid (KA) treatment of HT22 cells. Data are presented as means ± SEM. ****P < 0.0001, one-way ANOVA with Tukey's post-hoc test. (i–i) Representative immunoblots (i) and quantification (j) of STAT1 in agonist (Con, AG1, and AG1+DMF groups) and inhibitor (Con, 6-An, and 6-An + NAC groups) groups after KA treatment of HT22 cells. Data are presented as means ± SEM. **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey's post-hoc test.