Fig. 4.
KCs and LSECs present particle-derived Ag resulting in moderate T cell expression of CD25 and secretion of immunomodulatory mediators. C57BL/6 mice (n = 3) were injected with 2 mg of PLG-OVA NPs. After 24 h, liver non-parenchymal cells were isolated and KCs and LSECs were sorted by MACS and co-cultured (105 cells) with naïve CD4 OT-II T cells (0.5 × 105 cells). Bone marrow-derived DCs and MØs were used as controls with and without the addition of soluble OVA peptide. (A) The efficiency of Ag presentation was measured by CD25 expression on live CD4 T cells using flow cytometry. (B) Soluble IL-10 and PGE2 were measured in the co-culture supernatants. Statistical differences were determined using one-way ANOVA with Tukey’s multiple comparisons tests. Nonsignificant differences are indicated by matching letters (p > 0.05). Error bars represent SD and data are representative of 5 (A) and 2 (B) independent experiments.