Fig. 6.

Adoptively transferred PLG-Ag-containing KCs are sufficient for inducing tolerance in the EAE model. SJL mice (n = 5) were injected with 2 mg of Cy5.5 labeled PLG-PLP. 24 h later, the liver non-parenchymal cells were isolated, and KCs were sorted based on particle-derived Cy5.5 fluorescence. Particle-containing KCs were adoptively transferred into naïve SJL mice (10⁶ cells per mouse). The mice were immunized 7 days later with PLP/CFA and scored by blinded observers. Statistical differences were determined by the Mann-Whitney test (two-tailed nonparametric test) over the period from day 10–34 (***p < 0.001). Error bars indicate SEM and data are representative of 2 independent experiments.