FIG. 2.

(A) The locations of the transposon insertions in the recombinant viruses. The transposon sequence is shown as a filled bar, while the coding sequence of each open reading frame is represented by an open arrow. The orientation of the arrow represents the direction of the translation and transcription predicted based on the nucleotide sequence (32). The numbers represent the sizes of the DNA fragments of the mutant viruses that contained the transposon sequence and were generated by digestion with HindIII (H), NotI (N), or EcoRI (E). (B) Southern blot analyses of the viral mutants. The DNA fractions were isolated from cells infected with the wild-type (WT) virus and different MCMV mutants. The DNA samples (20 μg) were digested with HindIII (H), NotI (N), or EcoRI (E); separated on 0.8% agarose gels; transferred to a Zeta-Probe membrane; and hybridized to a DNA probe. The probes used for the analyses were the plasmids that contained the MCMV DNA fragments inserted with the transposon sequence.