FIG. 8.

The stability of the transposon mutations in tissue cultured cells and in BALB/c and SCID mice. Viral DNAs were either isolated from cells that were infected with Rvm09 (MOI = <0.01) and allowed to grow in culture for 5 days (P0) (lane 2) or five generations (60 days) (P5) (lane 3) or from cells that were infected with the virus collected from the salivary glands (SG, lanes 4 and 6) and lungs (LU, lanes 5 and 7) of either BALB/c (BALB/c, lanes 4 and 5) or SCID mice (SCID, lanes 6 and 7) 14 days after intraperitoneal inoculation with 10⁴ PFU of Rvm09. Southern blot analyses of the viral DNA fractions digested with HindIII are shown. The DNA of the wild-type virus (WT) is shown in lane 1. The ³²P-radiolabeled probe was derived from the same plasmid which was used for Southern analyses of Rvm09 in Fig. 2 and contained the transposon and the m09 open reading frame sequence.