FIG. 1.
Schematic representation of M-MuLV Gag proteins. The M-MuLV Pr65gag precursor protein consists of four domains, matrix (MA), p12, CA, and NC, which are cleaved by the viral protease during maturation. The gag gene contains 538 codons, and the N-terminal methionine of the precursor polypeptide is replaced with a myristyl group during or shortly after synthesis. The modified protein, His-MoCANCexact, expresses the polypeptide CANC regions corresponding to gag codons 213 to 538 and an amino-terminal polyhistidine tag of MGSSH HHHHH SSGLV APRGS HMLGD. His-MoCANC is composed of the His tag, the polypeptide CANC region corresponding to codons 213 to 523, and a carboxy-terminal tail, ADPAA NKARK EAELA AATAEQ. His-MoCA contains an N-terminal polyhistidine tag, the region corresponding to M-MuLV gag codons 213 to 478 and a C-terminal tail of ADPAA NKARK EAELA AATAEQ.