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. 2000 Aug;74(16):7431–7441. doi: 10.1128/jvi.74.16.7431-7441.2000

FIG. 4.

FIG. 4

In vitro assembly of protein-RNA complexes. His-MoCANC-plus-RNA complexes were assembled, transferred to EM grids, stained, and photographed as described in Materials and Methods and are shown with protein areas appearing white. Samples contained 12 μM His-MoCANC under the following conditions: (A) RNA pPsi4Pinawt (4,200 nt; 180 μM total nucleotide concentration), 16-h incubation at 30°C, photographed at ×27,500 (bar, 87 nm); (B) RNA pPsi4PinaΔPsi (3,980 nt; 180 μM total nucleotide concentration), 16-h incubation at 30°C, photographed at ×27,500 (bar, 924 nm); (C) RNA pPsi4PinaΔPsi (3,980 nt; 180 μM total nucleotide concentration), 16-h incubation at 30°C, photographed at ×52,000 (bar, 46 nm); (D) RNA pMMΔCD (680 nt; 78 μM total nucleotide concentration), 16-h incubation at 4°C, photographed at ×60,000 (bar, 318 nm); (E) RNA pMMΔCD (680 nt; 78 μM total nucleotide concentration), 16-h incubation at 4°C, photographed at ×11,000 (bar, 2,309 nm); (F) RNA pMMΔCD (680 nt; 78 μM total nucleotide concentration), 16-h incubation at 4°C, photographed at ×52,000 (bar, 488 nm).