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. 2000 Aug;74(16):7431–7441. doi: 10.1128/jvi.74.16.7431-7441.2000

FIG. 5.

FIG. 5

Tubes formed by His-MoCANC plus RNA. (A) His-MoCANC-plus-RNA tube segment. Shown is a section of a His-MoCANC-plus-RNA tube (tube 353-3) with protein areas appearing white. The apparent tube diameter (width) is 774 Å. (B) Power spectrum of the His-MoCANC-plus-RNA tube. The tube segment from panel A was Fourier transformed and is displayed as a power spectrum with the same dimensions as in the original image. The right-pointing arrowhead indicates a layer line at 1/65.7Å, and the left-side reflection on this line defines a lattice vector of 1/64.5Å. The left-pointing arrowhead indicates a layer line at 1/38.6Å, and the right-side reflection on this line defines a lattice vector of 1/37.8Å. (Both lattice vectors were derived after correction for the diffraction pattern rectangularity.) All His-MoCANC-plus-RNA tube diffraction patterns that could be indexed used lattice vectors corresponding to those shown (n = 75), although with some diffraction patterns these corresponded to the 3 and 5 layer lines (n = 33) while in others they corresponded to the 4 and 7 layer lines (n = 42). The average reciprocal space lattice dimensions for measured diffraction patterns were 1/a* = 63.8 ± 1.5 Å, 1/b* = 37.2 ± 1.2 Å, γ* = 22.6 ± 4.4° (n = 35). (C) Filtered image of the His-MoCANC-plus-RNA tube. A Fourier-filtered image of panel A was generated by masking reflections from panel B and applying this mask to the Fourier transform prior to backtransformation. As in panel A, protein areas appear white. (D) Image of one side of the His-MoCANC-plus-RNA tube. A Fourier-filtered image of one side of the tube segment in panel A was generated by masking the subset of reflections from panel B defined by the indicated lattice vectors, followed by filtration and backtransformation. The inset shows a magnification in which the inset width corresponds to 106 Å. As in panel C, protein areas appear white. Note that since the handedness of the tubes has not been defined, it is unclear whether the image represents the proximal or distal surfaces of the tube.