TABLE 1.

RNA binding abilities of His-MoCANC bound to membranes^a

RNA typeb	Amt of RNA (cpm) bound to proteins assembled on monolayersc
	MoCA	MoCANC	MoCANCexact	No protein
pGEM4	654	2,755	NDd	432
pMM2ΔPsi	102	1,948	2,104	ND
pMMΔCD	558	4,861	4,020	668
pMMwt	623	3,181	2,210	ND
GTP	360	497	ND	399

^aDrop incubations (10 μl) containing proteins (10 pmol) and 1 pmol (nucleotide concentration) of the indicated radiolabeled RNAs or GTP (specific activity, 2.2 × 10⁵ cpm/pmol of nucleotide; except GEM4 RNA, which was 8.8 × 10⁴ cpm/pmol of nucleotide) were overlaid with 1 μl of lipid monolayers composed of PC and Ni²⁺-DHGN, as described in Materials and Methods. After overnight incubations, monolayers with adherent proteins and RNAs were transferred to EM grids, which were washed, and bound radioactive materials were counted. 

^bApproximate RNA sizes were as follows: pGEM4, 60 nt; pMM2ΔPsi, 420 nt; pMMΔCD, 680 nt; pMMwt, 730 nt. 

^cValues are listed as cpm bound. 

^dND, not done.