TABLE 2.
Characteristicb | Circles | Tubes |
---|---|---|
Frequency (per 100 μm2) | 31–1,471 | 0.6–60 |
Diameter (Å) | 737 ± 69 (n = 79) | 686 ± 81 (n = 86) |
Wall thickness (Å) | 172 ± 19 (n = 103) | 174 ± 22 (n = 91) |
Length (nm) | NAc | 873 ± 453 (n = 118) |
RNA size (nt) | 424–4,200 | 678–4,200 |
No. of samples (grids) | 8 | 6 |
Fourteen EM grids, representing separate RNA-plus-His-MoCANC or His-MoCANCexact incubations performed as described in Materials and Methods, were examined for the presence of circle and tube morphological forms. Electron micrographs were obtained at magnifications of ×5,000 to ×75,000 and scanned for analysis.
Frequencies of morphological forms derive from areas of approximately 125 μm2 for circle forms and 300 μm2 for tube forms. Diameters, wall thicknesses, and lengths (for tube forms) were averaged from scanned images, where n indicates the number of individual circles or tubes contributing to the value. Note that the term “circle” is used here to describe the appearance of structures in 2D projections and that the actual 3D morphology of circles may be a spherical form. Note also that since morphological forms did not appear to vary with protein or RNA type, compiled values include results from incubations with His-MoCANC and His-MoCANCexact and with in vitro-transcribed RNAs of 424 to 4200 nt.
NA, not applicable.