FIG. 5.

Superinfection of two SIVmac-infected macaques with autologous SIV-infected cell lines resulted in an increase in plasma SIV RNA (A), increased numbers of CD4⁺ T cells (B), and an associated increased dominance of selected CDR3-bearing CD4⁺ T cells (C). Each macaque was inoculated intravenously with 3 × 10⁷ CD4⁺ T cells (10⁹ copies of SIV RNA in 10⁶ cells). Plasma RNA quantitation was done by QC-PCR as described elsewhere (30). Absolute numbers of CD4⁺ PBLs were calculated from data generated by flow cytometry analyses and complete blood counts, whereas those of lymph node CD4⁺ T cells (LN.C) were derived from flow cytometry data and total lymphocyte counts of a single lymph node (17). The spectrotyping data are displayed as described in the legend to Fig. 2.