FIG. 2.

γHV68-cyclin.LacZ grows similarly to wt γHV68. NIH 3T12 cells were infected with 5.0 (A) or 0.05 (B) PFU of wt γHV68 (closed squares) or v-cyclin.LacZ (open circles) per cell, samples were harvested at the times indicated, and virus was quantitated by plaque assay. Data are representative of two independent experiments. The dotted line indicates the sensitivity of the plaque assay (50 PFU). The inoculum was removed 1 h postinfection and replaced with fresh medium to measure single-step growth (A) or was left in place to measure multistep growth (B). (C) Viral growth in MEFs. MEF monolayers in 96-well plates were infected with 1 PFU (MOI, ca. 0.0001) of wt γHV68 or v-cyclin.LacZ. The CPE in 24 wells per virus was recorded over the course of 15 days postinfection. Results are the means of values for four independent dilution series of wt γHV68 and v-cyclin.LacZ (the standard errors of the means [SEM] are indicated by error bars). (D) Six wells of MEFs infected with 1 PFU of wt γHV68 or v-cyclin.LacZ were harvested at days 6, 8, and 14 for determination of viral titer by plaque assay. Results are the means of values for six wells each of wt γHV68 and v-cyclin.LacZ at each time point (SEMs are indicated by error bars).