Fig. 2.

Heterogeneity of mural cells in the acute phase of ischemic stroke. a Re-clustering analysis on pericytes and smooth muscle cells reveals 8–10 different subclusters depending on the timepoint and experimental condition. The pericyte subcluster 5, stroke-specific, is highlighted. b Dot plot representing the marker expression of each mural cell subcluster. Different timepoints and hemispheres are denoted by colors. The size of the dots equals the percentage of the population expressing the marker, and the intensity of the color denotes the expression level, respectively. Pericytes are defined as Pdgfrβ⁺, RGS5⁺, Atp13a5⁺, Abcc9⁺, and Acta2^-, SMCs Acta2⁺, fibroblasts Pdgfrβ⁺, Pdgfrα⁺. Clusters 6, 8, and 10 are pericytes co-expressing markers of pericytes and microglia (Aif1⁺), endothelial cells (Pecam1⁺), or oligodendrocytes (Mog⁺). c contralateral; i ipsilateral; h hour