Table 2.

Gene expression after complex I inhibition by MPP in LUHMES cells

Gene	Fold change	p value	RPKM baseline	Gene name
Glycolysis
GCK	3.16	1E − 31	2	Glucokinase
HK1	2.49	4E − 33	29	Hexokinase 1
HK2	3.72	6E − 72	3	Hexokinase 2
GPI	3.25	3E − 48	13	Glucose-6-phosphate isomerase
PFKM	1.89	6E − 14	24	Phosphofructokinase, muscle
ALDOA	3.92	4E − 62	45	Fructose-bisphosphate aldolase A
ALDOC	4.23	5E − 75	11	Fructose-bisphosphate aldolase C
TPI1	2.82	1E − 37	264	Triosephosphate isomerase 1
GAPDH	2.93	3E − 36	642	Glyceraldehyde-3-phosphate dehydrogenase
PGAM2	2.82	3E − 08	2	Phosphoglycerate mutase 2 (muscle)
ENO1	2.17	4E − 25	157	Enolase 1
ENO2	2.38	5E − 35	30	Enolase 2
ENO3	5.03	9E − 105	31	Enolase 3
PKM	1.49	5E − 07	91	Pyruvate kinase M1/2
Citric acid cycle
CS	3.53	4E − 65	31	Citrate synthase
ACO2	1.95	3E − 17	33	Aconitase 2
IDH1	1.40	7E − 07	53	Isocitrate dehydrogenase 1 (NADP +)
IDH2	1.93	9E − 17	23	Isocitrate dehydrogenase 2 (NADP +)
IDH3B	1.93	4E − 18	28	Isocitrate dehydrogenase 3 (NAD +) non-catalytic subunit β
IDH3G	1.67	1E − 08	13	Isocitrate dehydrogenase 3 (NAD +) non-catalytic subunit γ
DLD	0.53	5E − 14	19	Dihydrolipoamide dehydrogenase
SUCLG1	3.14	4E − 47	18	Succinate-CoA ligase subunit α
SUCLG2	2.01	1E − 08	2	Succinate-CoA ligase [GDP-forming] subunit β
SDHA	1.73	7E − 17	10	Succinate dehydrogenase complex flavoprotein subunit A
SDHB	1.80	2E − 13	23	Succinate dehydrogenase complex iron sulfur subunit B
FH	2.81	5E − 36	25	Fumarate hydratase
MDH2	2.71	4E − 36	44	Malate dehydrogenase 2, mitochondrial
Pentose phosphate pathway
G6PD	1.75	9E − 14	56	Glucose-6-phosphate dehydrogenase
PGLS	2.13	1E − 14	11	6-Phosphogluconolactonase
PGD	3.16	3E − 45	117	Phosphogluconate dehydrogenase
TKT	8.63	4E − 153	24	Transketolase
Citrate shuttle
CS	3.53	4E − 65	31	Citrate synthase
SLC25A1	4.11	9E − 78	16	Solute carrier family 25 member 1
ACLY	1.47	2E − 09	146	ATP citrate lyase
MDH2	2.72	4E − 36	44	Malate dehydrogenase 2, mitochondrial
ME1	7.78	2E − 97	1	Malic enzyme 1, cytosolic
ME2	1.79	3E − 18	7	Malic enzyme 2, mitochondrial
MPC2	2.89	4E − 31	7	Mitochondrial pyruvate carrier 2
Fatty acid synthesis
ACACA	2.77	1E − 53	11	Acetyl-CoA carboxylase α
ACSL1	0.44	3E − 26	12	Long-chain-fatty-acid—CoA ligase 1
ACSL3	2.07	8E − 11	18	Long-chain-fatty-acid—CoA ligase 3
ACSL4	1.92	7E − 11	12	Long-chain-fatty-acid—CoA ligase 4
ELOVL1	2.10	2E − 18	5	ELOVL fatty acid elongase 1
ELOVL5	2.69	4E − 57	35	ELOVL fatty acid elongase 5
ELOVL6	1.69	8E − 08	11	ELOVL fatty acid elongase 6
HACD2	0.46	2E − 14	3	Very-long-chain 3-hydroxyacyl-CoA dehydratase 2
TECR	1.68	3E − 11	14	Trans-2,3-enoyl-CoA reductase
SCD	7.78	8E − 255	160	Stearoyl-CoA desaturase
SCD5	1.73	1E − 12	6	Stearoyl-CoA desaturase 5
Carnitine shuttle (n.a.)
Fatty acid oxidation
ACADM	0.40	1E − 18	13	Acyl-CoA dehydrogenase, medium chain
ACADVL	2.16	9E − 23	20	Acyl-CoA dehydrogenase very long chain
ACAD9	0.50	1E − 13	8	Acyl-CoA dehydrogenase family member 9
HADH	4.14	1E − 67	6	Hydroxyacyl-CoA dehydrogenase
HADHA	1.69	9E − 15	53	Trifunctional enzyme subunit α
HADHB	1.99	7E − 23	20	Trifunctional enzyme subunit β

Sampling of all significantly induced or repressed genes (p < 10⁻⁶; n = 3) with robust baseline expression (RPKM > 1) for the following metabolic pathways: glycolysis, citric acid cycle, pentose phosphate pathway, citrate shuttle, fatty acid synthesis, carnitine shuttle, and fatty acid oxidation. A complete list of genes irrespective of their p value is provided in Suppl. Tab. 1, including a sampling of all detected NADPH-generating enzymes. n.a., not applicable (no significant changes occurred)