Table 4.
Gene expression changes following methionine restriction in rats
| Liver | Adipose | Muscle | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene | FC | p value | Gene | FC | p value | Gene | FC | p value | |
| Glycolysis and glucose uptake | GCK | 0.55 | 9E − 04 | LDHA | 1.74 | 2E − 03 | LDHB | 2.58 | 3E − 02 |
| PKLR | 0.62 | 2E − 03 | PFKM | 1.65 | 4E − 02 | ||||
| SLC2A4 | 1.89 | 8E − 04 | |||||||
| Citric acid cycle | ACO2 | 1.57 | 3E − 07 | ACO2 | 1.56 | 9E − 04 | |||
| IDH3A | 1.76 | 1E − 04 | |||||||
| MDH2 | 1.57 | 3E − 04 | |||||||
| Pentose phosphate pathway and NADPH provision | ME1 | 1.70 | 6E − 03 | ||||||
| MTHFD2 | 4.54 | 1E − 06 | |||||||
| Citrate shuttle | ACLY | 0.48 | 1E − 03 | ACLY | 1.68 | 1E − 02 | |||
| ME1 | 1.70 | 6E − 03 | MDH1 | 1.57 | 9E − 04 | ||||
| PC | 1.71 | 1E − 03 | |||||||
| Fatty acid synthesis and provision | ACACB | 0.46 | 2E − 07 | ACACA | 3.35 | 3E − 06 | |||
| ELOVL5 | 0.65 | 3E − 05 | ELOVL6 | 4.04 | 3E − 05 | ELOVL6 | 2.04 | 5E − 02 | |
| SCD | 0.07 | 5E − 06 | SCD | 2.66 | 2E − 04 | ||||
| ACSL1 | 1.72 | 4E − 03 | ACSL3 | 0.58 | 7E − 03 | ||||
| HSD17B12 | 1.79 | 5E − 05 | |||||||
| LIPE | 1.71 | 1E − 02 | |||||||
| MGLL | 1.61 | 2E − 02 | |||||||
| Carnitine shuttle | CPT1B | 1.80 | 1E − 03 | ||||||
| Fatty acid oxidation and uptake | CD36 | 1.78 | 6E − 04 | APOBR | 0.62 | 1E − 02 | CD36 | 1.71 | 3E − 02 |
| ACOT2 | 1.66 | 1E − 05 | ACAA2 | 1.55 | 1E − 02 | ||||
| HADHB | 1.62 | 5E − 04 | ACSF2 | 1.52 | 4E − 03 | ||||
| VLDLR | 5.79 | 2E − 09 | LIPE | 1.68 | 6E − 03 | ||||
| LPL | 1.75 | 2E − 02 | |||||||
Complete sampling of all significantly (p < 0.05) and parametrically (FC > 3/2 or FC < 2/3) altered metabolic genes in three tissues (liver, inguinal adipose tissue, quadriceps muscle) of methionine-restricted rats as per microarray experiment (n = 6). The following metabolic pathways were sampled: glycolysis and glucose uptake, citric acid cycle, pentose phosphate pathway and NADPH provision, citrate shuttle, fatty acid synthesis and provision, carnitine shuttle, and fatty acid oxidation and uptake. Italics indicate the significant modulation of a gene or one of its paralogues in more than one tissue. Hormone-sensitive lipase (LIPE) has divergent functions in adipose tissue (provision of fatty acids for export) and skeletal muscle (provision of fatty acids for local oxidation). Gene names are used as in Table 2 and Suppl. Tab. 1; additional genes were: LDHA/B, lactate dehydrogenase A/B; PKLR, pyruvate kinase L/R; SLC2A4, facilitated glucose transporter member 4 (GLUT4); LIPE, lipase E, hormone-sensitive; MGLL, monoglyceride lipase; CD36, fatty acid translocase; APOBR, apolipoprotein B receptor; ACOT2, acyl-CoA thioesterase 2; ACAA2, acetyl-CoA acyltransferase 2; ACSF2, acyl-CoA synthetase family member 2; MTHFD2, methylenetetrahydrofolate dehydrogenase/cyclohydrolase; VLDLR, very low-density lipoprotein receptor; LPL, lipoprotein lipase. FC, fold change