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. 2024 Jul 5;15(7):484. doi: 10.1038/s41419-024-06870-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Flow cytometry analysis of 2-NBDG-FITC-A and cell proportion at 48 h after cell attachment in CC group with low glucose or Calcitriol treatment. B Quantitative statistics of 2-NBDG-FITC-A intensity in (A). * CCHuh7R (Low glucose) vs CCHuh7R (Control) (p < 0.05); # CCHuh7m (Low glucose) vs CCHuh7m (Control) (p < 0.05); ns CCHuh7R (Calcitriol) vs CCHuh7R (Control) (p > 0.05); NS CCHuh7m (Calcitriol) vs CCHuh7m (Control) (p > 0.05). C Quantitative statistics of ratio of m + /m- in (A). D Flow cytometry analysis of mitochondrial membrane potential (Rhodamine123-FITC-A) and cell proportion at 48 h after cell attachment in CC group with low glucose or Calcitriol treatment. E Quantitative statistics of 2-NBDG-FITC-A in (D). ns CCHuh7R (Low glucose) vs CCHuh7R (Control) (p > 0.05); NS CCHuh7m (Low glucose) vs CCHuh7m (Control) (p > 0.05); * CCHuh7R (Calcitriol) vs CCHuh7R (Control) (p < 0.05); # CCHuh7m (Calcitriol) vs CCHuh7m (Control) (p < 0.05). F Quantitative statistics of ratio of m + /m- in (D). G Flow cytometry analysis of cell death (SYTOX Green-FITC-A) and cell proportion at 48 h after cell attachment in CC group with low glucose or Calcitriol treatment. H Quantitative statistics of Dead m + /m+ ratio in (G). I Quantitative statistics of m + /m+ ratio in (G). J High content imaging of cell growth at 0, 24 h, 48 h after cell attachment in CC group with low glucose or Calcitriol treatment using 10X objective lens. K Quantitative statistics of cell number in (J) (Details see Video 11–13). Three independent experiments were conducted, and the values are represented by means ± SEM using two-way ANOVA with Tukey’s multiple comparisons test (B, E and K) or Ordinary one-way ANOVA with Sidak’s multiple comparisons test (C, F, H and I). *p < 0.05, #p < 0.05, ns/NS non-statistically significant.