Skip to main content
. 2024 Jun 13;74:103230. doi: 10.1016/j.redox.2024.103230

Fig. 6.

Fig. 6

AKG pretreatment inhibits the ubiquitination of endogenous Nrf2 in lipid-overloaded mouse liver. (A) Western blot image and (B) Statistical analysis on the relative protein content of Nrf2 in the cytoplasm and nuclear in the liver. (C) Western blot image and (D) Statistical analysis on the relative protein content of Nrf2 in the cytoplasm and nuclear in the HepG2 cells. (E) Immunoprecipitated Nrf2 in liver tissue then detect the ubiquitination of endogenous Nrf2 by Immunoblot analysis. Add 20 μM MG132 to cells 6 h before collection. (F) Immunoprecipitated Nrf2 in HepG2 cells then detect the ubiquitination of endogenous Nrf2 by Immunoblot analysis. The values are means ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.