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Binding of infectious WR and vH3Δ to cells. Replicate wells of chilled BS-C-1 cells were inoculated with 1 PFU of unpurified WR or vH3Δ per cell and maintained on ice. At 1, 10, 20, 30, 45, and 60 min, cells were washed with cold PBS, harvested, dispersed, and plated on fresh BS-C-1 monolayers at 37°C. After 48 h, the cells were stained with crystal violet and the plaques were enumerated.
