Table 3: Comparison of the relative catalytic velocities of ENPP1 variants with prior studies.

The relative catalytic velocities determined herein (Ansh activity) are listed compared with prior studies. There is good concordance with prior measurements with two exceptions: L611V and R481W. See text for discussion.

Pathogenic Variant	Ansh activity	Prior activity 1	Ref. activiy 1	Prior activity 2	Ref. activity 2
C126R	0%	3%	[52]
K173Q	63%	66%	[47]	83%	[52]
C195R	0%	0%	[47]
Y301C	49%	0%	[47]
P305T	4%	0%	[47]
R456Q	2%	11%	[52]
Y471C	33%	43%	[52]
R481W	68%	37%	[52]
S504R	8%	51%	[47]
L579F	0%	0%	[52]
L611V	111%	0%	[52]
Y659C	11%	41%	[47]
E668K	74%	100%	[47]	70%	[52]
R774C	26%	57%	[47]	36%	[52]
H777R	9%	38%	[47]
N792S	26%	17%	[52]
R821H	107%	88%	[47]
R888W	4%	0%	[47]
K905fsX15	0%	0%	[52]