FIG. 6.

Effect of MAb K41 and FIP on CDV N mRNA levels under conditions allowing virus spread by cell-cell fusion. Vero cells were infected with CDV strain OND-SP at a low MOI of 0.1. MAb K41 (12 μg/ml) alone, FIP (200 μg/ml) alone, or combinations of the two were added to the cultures after 12 h as indicated. RNA was harvested at 0, 6, 12, 18, and 24 hpi, blotted on Hybond-N filters, and hybridized to ³²P-labeled probes for CDV N (A) and GAPDH (B). Mean values of the N/GAPDH signal ratio are given in panel C.