Fig. 1.
Comparison of the characteristics of ex vivo-expanded NG2/BMMSCs and parentalBMMSCs. (Aa-c) Comparison of the sizes of m-NG2/BMMSCs (a) and parental m-BMMSCs (b) and quantification (c, n = 20). (B) FCM analyses and IF staining (red) of NG-2+ cells (arrow) in mouse marrow MSC (m-BMMSC) cultures; n = 6/type of experiment. (C) Measurement of the purity of the isolated and ex vivo-expanded NG2+ cells using FCM (left panel) and IF staining (green, arrow); n = 3/method. (D) NG2+ cells (boxes) within m-BMMSC cultures generated smaller spindle-shaped BMMSCs (thin arrows, S1 and S2 represent individual cultures. (Ea, b) IF staining for SSEA-3 in m-NG2/BMMSCs (a, red) and m-BMMSCs (b, green) and quantification from a and b. (F, boxes), n = 3. The means ± SDs of duplicate preparations from three independent experiments are shown. Scale bars = 200 μm for all the images except for those in A and D; scale bars = 100 μm. ** ##p < 0.001 compared with m-BMMSCs
