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. 2024 Jul 6;15:199. doi: 10.1186/s13287-024-03817-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Proliferation potential of the ex vivo-expanded m-NG2/_BMMSCs in normal cultures. (A, B) A CCK-8 assay for comparison of the two types of cells in growth rates from normal cultures at 24, 48, and 72 h. (Ca, b) Comparison of growth rates at 5 and 6 days (a) and quantification (b, boxes); n = 3/time point. (Da, b) Double IF staining to identify m-NG2/_BMMSCs (a, NG2, red) costained with Ki-67 (green, a, arrows), and the same procedure was used for parental _BMMSCs (b, CD9, red, arrows); n = 6. (E) Quantification of the percentage of Ki-67-positive cells in D (merged, boxes) after 72 h in cultures; n = 6. (F) Fold changes in the expression of Ki-67 in m-NG2/_BMMSCs over that in _BMMSCs were analyzed as described in (E). The means ± SDs of duplicate preparations from three independent experiments are shown. Scale bars: Ca = 100 μm, others = 200 μm. * p < 0.05 and **p < 0.001 compared with m-_BMMSCs