Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 6;15:199. doi: 10.1186/s13287-024-03817-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Direct differentiation of BDCs from m-NG2/_BMMSCs in response to DEN-induced liver injury cues. (Aa-b) The morphology of m-NG2/_BMMSCs in control medium (Ctrl-CM, a) and in the _DENCM (b), bold arrows indicate assumed NG2⁺ cells, red arrows indicate as bile duct-like cells. Little change was observed in parental m-_BMMSCs during this period (b, right panels) compared to that in Ctrl-CM (a, right panel): S1, S2 in b represent individuals, scale bar = 100 μm; remaining panels: scale bar = 200 μm. (Ba, b) Double IF staining for NG2⁺ cells (red) or CD9⁺ cells (green) covered by CK19⁺ cells (green/red) of m-NG2/_BMMSCs (a) and m-_BMMSCs (b) cultured in _DENCM for 18–24 h. (C) Quantification (Ba, b) of the merged cells. (Da-d) IF staining (red) for host Lyve-1⁺ cell expression (boxes) in subgroups of liver sections. (Ea, b) Quantificative analysis of Lyve-1 expression in subgroups from boxes in Da-d (a) and the number of vessel-like structures formed from the groups in Dc, d (b, n = 6). (Fa-d) Direct differentiation of Lyve-1⁺ cells from donor m-NG2/_BMMSCs (a) and parental m-_BMMSCs (b) in _DENCM and comparative quantification of total numbers per quarter area (boxes, c) and the number of vessel-like formations (red arrows, d, n = 6). At least three independent experiments were performed, and the data are presented as the means ± SDs. Scale bar = 200 μm. #p < 0.05 compared with naive; **p < 0.001 compared with m-_BMMSCs; ns: no significance compared with m-_BMMSCs