Table 2.
Antibodies (abs), reagents, and kits
| Item | Antibody | Cat. No. | Application(s) | Company | City, State | Country |
|---|---|---|---|---|---|---|
| Primary Abs | Rabbit Polyclonal Anti-NG2 | AB5320B | Immunofluorescence, Western blot analysis | Millipore | Massachusetts | USA |
| Rabbit Polyclonal Anti-SSEA-3 | bs-3575R | Bioss | Beijing | China | ||
| Rabbit Polyclonal Anti-MDR1 | bs-0563R | |||||
| Rabbit Polyclonal Anti-Ki-67 | bs-2130R | |||||
| Mouse Monoclonal Anti-CK19 | sc-374,192 | Santa Cruz Biotechnology | California | USA | ||
| Mouse Monoclonal Anti-α-SMA | sc-130,617 | |||||
| Mouse Monoclonal Anti-VWF | sc-365,712 | |||||
| Mouse Monoclonal Anti-CD31 | 102,401 | Biolegend | California | USA | ||
| Rabbit Polyclonal Anti-ZO-1 | 21773-1-AP | Proteintech | Wuhan | China | ||
| Rabbit Polyclonal Anti-CFTR | 20738-1-AP | |||||
| Rabbit Polyclonal Anti-E-cadherin | 20874-1-AP | |||||
| Rabbit Polyclonal Anti-Beta catenin | 17565-1-AP | |||||
| Rabbit Polyclonal Anti-AQP1 | 20333-1-AP | |||||
| Rabbit Polyclonal Anti- Collagen Type I | 14695-1-AP | |||||
| LYVE1 | 14-0443-80 | Invitrogen | California | USA | ||
| Secondary antibodies | Cy3 AffiniPure Goat Anti-Rabbit IgG (H + L) | 111-165-003 | Immunofluorescence | Jackson ImmunoResearch | Pennsylvania | USA |
| Alexa Fluor 488 AffiniPure Goat Anti-Rabbit IgG (H + L) | 111-545-003 | |||||
| Alexa Fluor 594 AffiniPure Donkey Anti-Mouse IgG (H + L) | 715-585-150 | |||||
| Cy3 AffiniPure Goat Anti-Mouse IgG (H + L) | 115-165-003 | |||||
| Alexa Fluor 488 AffiniPure Goat Anti-Mouse IgG (H + L) | 115-545-003 | |||||
| Antibodies for FCM | Alexa Fluor 488-Mouse-NG2 | 562,413 | Flow Cytometry (FCM) | BD Biosciences | California | USA |
| FITC-Mouse-CD9 | 124,808 | Biolegend | California | USA | ||
| PE-Mouse-PDGFR-β | 136,005 | |||||
| APC-Mouse-CD105 | 120,414 | |||||
| PE-Mouse-CD31 | 102,407 | |||||
| PE Rat IgG2a, κ Isotype Ctrl Antibody | 400,507 | |||||
| FITC-Human-CD34 | AH0134225 | Precision BioMedicals | Chongqing | China | ||
| PE-Human-CD73 | AH0273125 | |||||
| Reagents | bGJb medium (Fitton–Jackson Modification) | 2,216,413 | Cell culture | Gibco | California | USA |
| DMEM/F-12 (1:1) basic (1X) | C11330500BT | |||||
| Percoll | LS-0073 | LANGTIAN | Chongqing | China | ||
| Poly- L -lysine | 25988-63-0 | Cell culture | Sigma‒Aldrich | Missouri | USA | |
| DEN | N0756 | Animal models | ||||
| DAPI | C0065 | Immunofluorescence | Solarbio | Beijing | China | |
| CFSE | 1,948,067 | Cell tracking | Invitrogen | California | USA | |
| Cell Tracker™ Red CM-DiI | C7000 | Cell tracking | Invitrogen | NY | USA | |
| Streptomycin | C0222 | Cell cultures | Beyotime, | Shanghai | China | |
| Kits | PrimeScript™ RT reagent Kit with gDNA Eraser | RR047A | RT‒qPCR | TaKaRa Bio, Inc. | Shiga | Japan |
| TB Green® Premix Ex Taq™ II | RR820A | |||||
| One Step TUNEL Apoptosis Assay Kit (Green Fluorescence) | C1086 | Cell apoptosis assay | Beyotime | Shanghai | China | |
| Enhanced BCA Protein Assay Kit | P0010 | Western blot analysis | ||||
| Enhanced Cell Counting Kit-8 | C0043 | Cell proliferation assay | ||||
| Hematoxylin Solution | ZLI-9610 | H&E | ZSGB-Bio | Beijing | China | |
| Eosin Dyeing Solution (alcohol-soluble) | BL703B | H&E | Biosharp | Anhui | China | |
| Modified Masson’s Trichrome Staining Kit | G1346 | Masson | Solarbio | Beijing | China |