Fig. 4.

ENT-A011 has positive effect on mouse cortical NSCs. A, A′ ENT-A011 increases proliferation on cortical NSCs, Representative fluorescence microscopy images of Immunostaining for BRDU and Nestin in adult hippocampal NSCs treated with BDNF or ENT-A011 for 24 h with or without Aβ42 (A). Quantification of BRDU fluorescence change with or without Aβ42 (A′). Quantification represents BRDU positive cells normalized against Nestin positive cells. (without Aβ42: N = 6, with Aβ42: N = 3), error bars represent S.E.M., Student’s t-test against Control; *p < 0.05, **p < 0.01, ***p < 0.001. B, B′ The compound rescues cortical NSCs from Aβ-induced cell death. Representative fluorescence microscopy images of Celltox cytotoxicity assay on hippocampal NSCs in the presence of Aβ42, treated with BDNF or ENT-A011, with or without the TrkB inhibitor ANA-12 for 24 h (B′ right). Quantification of fluorescence change and MTT levels after BDNF or compound treatment, with or without ANA-12 (without ANA-12: N = 3–5, with ANA-12: N = 5) (B′ left). Quantification represents Celltox positive cells normalized against Hoechst positive cells. (without ANA-12: N = 5–7, with ANA-12: N = 3–5), error bars represent S.E.M., Student’s t-test against Control; *p < 0.05, **p < 0.01, ***p < 0.001. Scalebars = 20 μm