Fig. 6.
ENT-A011 effect on human cortical Neural Progenitor Cells (NPCs). Change in BRDU + cells (calculated as percentage of total Hoechst + cells) after BDNF or ENT-A011 treatment for 48 h in NPC derived from 3 human induced pluripotent stem cell (iPSC) lines. Representative fluorescence microscopy images of Immunostaining for BRDU and Hoechst (A) and quantification of BRDU fluorescence change (A′). B, B′ NPC derived from 3 human iPSC lines were treated with Aβ1-42 oligomers and BDNF or ENT-A011 for 48 h and compound effect on reducing toxicity was assessed via the Celltox cytotoxicity assay. Representative fluorescence microscopy images of Celltox cytotoxicity assay in the presence of Aβ42, treated with BDNF or ENT-A011 for 24 h (B). Quantification of fluorescence change and MTT levels after BDNF or compound treatment, with or without ANA-12 (B′). Quantification represents Celltox positive cells normalized against Hoechst positive cells. Three different lines were differentiated, N = 3–4, error bars represent S.E.M., Student’s t-test against Control; *p < 0.05, **p < 0.01, ***p < 0.001. Scalebars = 20 μm