FIG. 6.

Sequences in the pentanucleotide region of vector construct progeny. ClampR fragments were obtained with primer set NS21 and NS22 (map positions 2240 to 2259 and 2382 to 2401, respectively) for progeny dsRNAs of vectors Ct2APH, Ct2APHp29Δ25-243, and Δp69bEGFP2A 9 weeks after transfection and sequenced. Nucleotide sequences and deduced amino acid sequences for the ORF A-ORF B junction of CHV1-EP713 are shown for reference. Asterisks indicate termination codons of ORF A, and the underlined sequence refers to the pentanucleotide. Nonviral nucleotides are shown in lowercase letters.