TABLE 1.
Properties of vector constructs
| Group and vector | Replication competencya | Green fluorescenceb | Stabilityc |
|---|---|---|---|
| I | |||
| p29Δ25-243EGFP | + | − | − |
| p29Δ25-243HYG | − | NAd | NA |
| p29Δ25-243EGFP2A | + | + | − |
| p29Δ25-109EGFP | + | + | − |
| p29Δ25-109HYG | − | NA | NA |
| p29Δ25-109EGFP2A | + | + | − |
| II | |||
| NtNcoI | + | NA | ++ |
| NtNcoIEGFP | − | NA | NA |
| NtNcoIEGFPp29Δ25-243 | − | NA | NA |
| NtNcoIEGFP2A | − | NA | NA |
| NtNcoI2AΔp29 | − | NA | NA |
| NtNcoIEGFP2AΔp29 | − | NA | NA |
| III | |||
| Ct2A | − | NA | NA |
| Ct2APH | + | NA | + |
| Ct2APHp29Δ25-243 | + | NA | + |
| Ctp40[2A] | + | NA | + |
| Ctp40[2AEGFP] | + | +++ | − |
| Ctp40[2AHYG] | − | NA | NA |
| IV | |||
| Δp69bEGFP | + | − | + |
| Δp69bEGFP2A | + | − | + |
a
Vector viability was examined based on virus-specific dsRNA recovery as shown in Fig. 2.
b
The ability of a vector virus to fluoresce is indicated as +++ (moderately highly fluorescent), + (faintly fluorescent), and − (negative).
c
The stability of vectors was analyzed by ClampR: ++, stable as long as 9 weeks posttransfection; +, stable as long as 3 weeks posttransfection; −, underwent deletion before 3 weeks posttransfection.
d
NA, not applicable.