Figure 2.

Improvements in exercise capacity following training are facilitated by Kvβ proteins. (A) Time course of 4 wk exercise training protocol used for wt and β2^−/− mice. (B) Compliance of wt and β2^−/− mice to the 4 wk exercise training protocol, recorded as either the percent of total sessions completed, or percent time (min) completed. ns: p > 0.05, Mann Whitney test; n = 10 mice each. (C) Total, lean, and fat body mass measured in wt and β2^−/− mice following completion of the 4 wk exercise training protocol. *p < 0.05, ns: p > 0.05, Mann Whitney test, n = 6 mice each. (D) Activity plots showing beam break counts recorded in 10 min intervals over a 24 h period in wt and β2^−/− mice following completion of exercise training protocol. **p < 0.01, two-way ANOVA. Inset shows 24 h summed activities measurements. ns: p > 0.05, unpaired t-test. (E) Mean (line) ± SE (shading) 24 h respiratory exchange ratio (VCO₂:VO₂) measured in wt and β2^−/− mice following completion of training protocol. p > 0.05, two-way RM ANOVA; n = 4 mice each. (F) Work performed to exhaustion in exercise capacity tests before (-) and after (exe) exercise training period in wt and β2^−/− mice. wt: *p < 0.05, β2^−/−: p = 0.06, paired t-test, n = 10 mice each. (G) Blood lactate concentrations measured at rest (before capacity test) and after fatigue in exercise trained wt and β2^−/− mice. ns: p > 0.05, two-way RM ANOVA, n = 10 mice each. (H) Work performed to exhaustion in exercise capacity tests in sedentary (sed) and exercised (exe) sm22α-rtTA and sm22α-rtTA:TRE-Kcnab1 transgenic mice. *p < 0.05, paired t-test, n = 10 mice each. (I) Blood lactate concentrations measured at rest (before capacity test) and after fatigue in sedentary and exercise-trained rtTA and rtTA:Kcnab1 mice. ns: p > 0.05, two-way RM ANOVA, n = 5–7 mice.