Figure 1.

Zinc binding sites in GlyRs. (A) Location of the proposed inhibitory (red) and potentiating (green) zinc binding sites within the ECD of GlyRs. The primary subunit is shown in white, and the complementary subunit is shown in gray. Black lines indicate the lipid membrane with the extracellular (Ex) and intracellular (In) leaflets labeled. (B) Potentiating zinc binding cavity of GlyRα₃ with co-ordinating residues shown in green. A zinc ion is shown in gray, and is coordinated by E192, D194 and H215. An aligned structure of GlyRα₁ with coordinating residues is shown in gray. (C) Proposed inhibitory binding site of GlyRα₃ viewed from the center of the receptor with important residues shown in red from the primary (dark) and complementary (light) subunit. An aligned structure of GlyRα₁ with coordinating residues is shown in gray. (D) Conservation of zinc binding residues in GlyR subunits. Non-conserved residues are highlighted in gray. Accession code for PBD file used: 5TIO (GlyRα₃) and 8DN4 (GlyRα₁).