Figure 2.

Zinc sensitivity of W170S mutation in GlyRα₁. (A) Example traces of zinc concentration-responses conducted on GlyRα₁ WT and GlyRα₁ W170S. The EC₅ concentration for GlyRα₁ WT is 5 μM and GlyRα₁ W170S is 12 μM. (B) Zinc concentration-responses are plotted for WT (black) and W170S (blue) GlyRα₁. Data is plotted as mean ± SEM with an n ≥ 5 and fitted using a bell-shaped dose response model. The modulatory activity at each concentration is compared between the two receptors using multiple t-tests. The degree of significance is denoted as: * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001 and **** = p ≤ 0.0001.