Figure 3.

Impact of zinc chelators on GlyRα₁. (A) Example traces illustrating the co-application of tricine (blue bars) and Ca-EDTA (red bars) on a current elicited by an EC₅ concentration of glycine (black bar) for WT (5 μM) and W170S GlyRα₁ (12 μM). Increasing concentrations of (A) tricine or (B) Ca-EDTA were co-applied with an EC₅ concentration of glycine on WT and W170S GlyRα₁. Data is normalized to the EC₅ concentration of glycine for each cell. Data is plotted as mean ± SEM with an n ≥ 5 and fitted using a modified Hill equation with a variable slope linear-regression model.