Figure 4.

Impact of the zinc insensitive W170S mutation on lipid modulation of GlyRα₁. C18 ω9 Glycine is represented as NOGly and 8–8 ortho phenylene glycine is represented as 8–8 OPGly. (A) Example traces illustrating the co-application of 1 μM NOGly or 1 μM 8–8 OPGly (red bars) on currents elicited by an EC₅ glycine concentration (black bar) on WT (5 μM) and W170S GlyRα₁ (12 μM). (B) Degree of potentiation induced by 1 μM NOGly and 1 μM 8–8 OPGly on WT (Gray) and W170S (Blue) GlyRα₁. Data is normalized to the degree of potentiation on WT GlyRα₁ and is compared using a t-test. Data is represented as mean ± SEM with an n ≥ 5. The degree of significance is denoted as: * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001 and **** = p ≤ 0.0001.