Figure 5.

Impact of zinc chelators on lipid modulation of GlyRα₁. 8–8 ortho phenylene glycine is represented as 8–8 OPGly. (A) Example traces illustrating the co-application of 8–8 OPGly (red bars) with an EC₅ concentration of glycine (black bars) in the absence and presence of tricine (blue bar) or Ca-EDTA (pink bar) for GlyRα₁ W170S. Raw current elicited by an EC₅ concentration of glycine in the absence (ND96:Gray) and presence of (B) 10 mM Tricine (Blue) or (D) 10 μM Ca-EDTA (Pink) conducted on the same cell. The EC₅ concentration for GlyRα₁ WT is 5 μM and GlyRα1 W170S is 12 μM. Data is normalized to the current in the absence of chelator and is compared using a paired t-test. Potentiation induced by a 1 μM concentration of 8–8 OPGly in the absence (ND96:Gray) and presence of (C) 10 mM Tricine (Blue) or (E) 10 μM Ca-EDTA (Pink) conducted on the same cell. Data is normalized to the lipid potentiation in the absence of chelator and is compared using a paired t-test. For all panels data is represented as mean ± SEM with an n ≥ 5. The degree of significance for statistical tests is denoted as: ns = not significant, * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001 and **** = p ≤ 0.0001.