Figure 6.

Synergistic interaction between zinc and 8–8 ortho phenylene glycine at GlyRα₁. 8–8 ortho phenylene glycine is represented as 8–8 OPGly. (A) Example trace illustrating the individual application of 1 μM zinc (blue bar) and 1 μM 8–8 OPGly (red bar) on a current elicited by an EC₅ concentration of glycine (black bar, 5 μM), and the co-application of both modulators. (B) Degree of potentiation induced by 1 μM zinc (blue), 1 μM 8–8 OPGly (red) or 1 μM of both modulators co-applied (pink) on the same cell. The sum of the two modulators applied individually is shown as the estimate (purple). The degree of potentiation in the co-application condition and the estimate are compared using a paired t-test. The degree of significance is denoted as: * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001 and **** = p ≤ 0.0001. (C) Potentiation induced in each condition. Data is represented as mean ± SEM with an n ≥ 5.