REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Polyclonal anti-KRT6A	Proteintech	Cat# 10590-1-AP; RRID: AB_2134306
Rabbit Polyclonal anti-S100A8	Proteintech	Cat# 15792-1-AP; RRID: AB_10666315
Rabbit Monoclonal anti-αSMA	Abcam	Cat# ab124964; RRID: AB_11129103
Rabbit Monoclonal anti-Collagen 1A1	Cell Signaling Technology	Cat# 72026; RRID: AB_2904565
Rabbit Monoclonal anti-FAK	Abcam	Cat# ab40794; RRID: AB_732300
Rabbit Monoclonal anti-phospho-FAK (Tyr 397)	Cell Signaling Technology	Cat# 8556; RRID: AB_10891442
Rabbit Monoclonal anti-Src	Cell Signaling Technology	Cat# 2123; RRID: AB_2106047
Rabbit Monoclonal anti-phospho-Src (Tyr 416)	Cell Signaling Technology	Cat# 6943; RRID: AB_10013641
Rabbit Polyclonal anti-CD31	Servicebio	Cat# GB11063-2-100; RRID: AB_2922436
Rabbit Polyclonal anti-CXCL1	Proteintech	Cat# 12335-1-AP; RRID: AB_2087568
Mouse Monoclonal anti-RhoA-GTP	NewEast Biosciences	Cat# 26904; RRID: AB_1961799
Rabbit Monoclonal anti-RhoA	Cell Signaling Technology	Cat# 2117; RRID: AB_10693922
Rabbit Monoclonal anti-ROCK1	Cell Signaling Technology	Cat# 4035; RRID: AB_2238679
Rabbit Monoclonal anti-phospho-MLC2 (Ser 19)	Cell Signaling Technology	Cat# 3671; RRID: AB_330248
Rabbit Monoclonal anti-MLC2	Cell Signaling Technology	Cat# 8505; RRID: AB_2728760
Rabbit Polyclonal anti-MRTF-A	Proteintech	Cat# 21166-1-AP; RRID: AB_2878822
Rabbit Polyclonal anti-CD147	Proteintech	Cat# 11989-1-AP; RRID: AB_2290597
Rabbit Monoclonal anti-phospho-ERK1/2 (Thr 202/Tyr 204)	Cell Signaling Technology	Cat# 4370; RRID: AB_2315112
Rabbit Monoclonal anti-ERK1/2	Cell Signaling Technology	Cat# 4695; RRID: AB_390779
Rabbit Monoclonal anti-cleaved-PARP (Asp 214)	Cell Signaling Technology	Cat# 5625; RRID: AB_10699459
Rabbit Monoclonal anti-cleaved-Caspase3 (Asp 175)	Cell Signaling Technology	Cat# 9661; RRID: AB_2341188
Rabbit Polyclonal anti-TFAP2A	Proteintech	Cat# 13019-3-AP; RRID: AB_2199414
Mouse Monoclonal anti-Vinculin	Proteintech	Cat# 66305-1-Ig; RRID: AB_2810300
Mouse Monoclonal anti-GAPDH	Proteintech	Cat# 60004-1-Ig; RRID: AB_2107436
Normal Rabbit IgG	Cell Signaling Technology	Cat# 2729; RRID: AB_1031062
Rabbit monoclonal anti-Histone H3	Cell Signaling Technology	Cat# 4620; RRID: AB_1904005
Goat anti-rabbit IgG, HRP-linked	Cell Signaling Technology	Cat# 7074; RRID: AB_2099233
Horse anti-mouse IgG, HRP-linked	Cell Signaling Technology	Cat# 7076; RRID: AB_330924
Bacterial and virus strains
S100A8 shRNA lentivirus	Genechem	N/A
S100A8 overexpression lentivirus	Genechem	N/A
CD147 Cas9-sgRNA lentivirus	Genechem	N/A
Biological samples
ESCC tumor specimens	Linzhou Cancer Hospital	See Table S1 for details
Chemicals, peptides, and recombinant proteins
DMEM	Corning	Cat# 10-013-CV
Advanced DMEM/F12	Gibco	Cat# 12634028
Fetal Bovine Serum	Cell Technologies	Cat# 30070
5-Fluorouracil	MCE	Cat# HY-90006
Cisplatin	MCE	Cat# HY-17394
Collagenase I	Gibco	Cat# 17100017
Collagenase IV	Gibco	Cat# 17104019
Hyaluronidase	Sigma-Aldrich	Cat# H1115000
B-27	Gibco	Cat# 17504044
HEPES	Gibco	Cat# 15630080
GlutaMAX™	Gibco	Cat# 35050061
EGF	Gibco	Cat# PMG8043
Y-27632	Selleck Chemicals	Cat# S1049
A83-01	Tocris Bioscience	Cat# 2939
Puromycin	Gibco	Cat# A1113803
Lipofectamine 2000	Invitrogen	Cat# 11668027
AC-73	MCE	Cat# HY-122214
Human recombinant S100A8 protein	Novoprotein	Cat# C794
Triton X-100	Solarbio	Cat# T8200
Critical commercial assays
RNA-Quick Purification Kit	ES Science	Cat# RN001
TB Green® Premix Ex Taq™II (Tli RNaseH Plus)	TaKaRa	Cat# RR820A
Masson’s Trichrome Stain Kit	Solarbio	Cat# G1340
Meilunbio® FGSuper Sensitive ECL Luminescence Reagent	Meilunbio	Cat# MA0186-1
Pierce™ Rapid Gold BCA	Thermo Fisher Scientific	Cat# A53226
Human S100A8/S100A9 ELISA Kit	Proteintech	Cat# KE00177
Opal 5-Color Manual IHC Kit	PANOVUE	Cat# 10144100100
SimpleChIP® Plus Sonication Chromatin IP Kit	Cell Signaling Technology	Cat# 56383
Deposited data
RNA-seq data of PDX mice	This paper	GSA: CRA013775
RNA-seq data of PDX donors	Yang et al.25	GSA: HRA004329
ESCC scRNA-seq data	Zhang et al.20	GEO: GSE160269
Experimental models: Cell lines
KYSE450	Dr. Y. Shimada	JCRB1430
KYSE510	Dr. Y. Shimada	JCRB1436
KYSE180	Dr. Y. Shimada	JCRB1083
Human esophageal cancer associated fibroblasts (ECAFs)	This paper	N/A
PDX-derived primary tumor cells (PDCs)	This paper	N/A
Experimental models: Organisms/strains
NSG mouse	BEIJING IDMO Co., Ltd	N/A
Oligonucleotides
shRNA sequences, see Table S2	Genechem	N/A
sgRNA sequences, see Table S2	Genechem	N/A
siRNA, see Table S2	JTSBIO Co.,Ltd (Wuhan, China)	N/A
qRT-PCR primers, see Table S3	Tsingke Biotechnology	N/A
Software and algorithms
R 4.2.2	R Core Team	https://www.R-project.org/
Trim Galore (version 0.6.6)	Felix Krueger	https://github.com/FelixKrueger/TrimGalore
Disambiguate (version 1.0)	Ahdesmäki et al.35	https://github.com/AstraZeneca-NGS/disambiguate
Salmon (version 1.2.0)	Patro et al.84	https://combine-lab.github.io/salmon/
DESeq2 (version 1.38.3)	Love et al.85	https://bioconductor.org/packages/DESeq2/
tximport (version 1.26.1)	Soneson et al.86	https://bioconductor.org/packages/tximport/
clusterProfiler (version 4.6.2)	Wu et al.87	https://github.com/YuLab-SMU/clusterProfiler
Seurat (version 4.3.0)	Hao et al.88	https://github.com/satijalab/seurat
Harmony (version 0.1.1)	Korsunsky et al.89	https://github.com/immunogenomics/harmony
CellChat (version 1.6.1)	Jin et al.90	https://github.com/sqjin/CellChat
survminer (version 0.4.9)	Kassambara et al.	https://github.com/kassambara/survminer
ImageJ	NIH	https://imagej.nih.gov/ij/
Prism 8.0	GraphPad	http://www.graphpad.com/