TABLE 3.

Inhibition coefficients of HIV-1 protease mutant (M46I, G48V, I50V, I84L)^a

Protease inhibitorb	ki (nM) for:		Mutant/ wild-type ratio
	Wild type	Mutant
APV	0.6	1.3	2
SQV	0.8	240	300
IDV	1	4	4
RTV	0.3	0.5	2
NFV	1.1	1.4	1

^aFor details, see reference 10. The substrate His-Lys-Ala-Arg-Val-Leu/p(NO₂) Phe-Glu-Ala-Nle-Ser-NH₂ is referred to as substrate 1 in reference 10. Assay conditions were as in the experiment in Table 2, except that the various PIs were allowed to preincubate with the wild-type or mutant protease and the reactions were initiated by addition of the substrate. K_i values were determined as described in the footnote to Table 2. 

^bIDV, indinavir; RTV, ritonavir; NFV, nelfinavir.