Fig. 4.
Antitumor response induced by CTLA-4 (post) is Treg-dependent. Mice were first treated with anti-CTLA-4 (clone 9H10) and then with CTLA-4 Ig (post). Dot-plot representation of (A) frequency and (B) absolute number of Foxp3+ CD4 T cells on day 24. Dot-plot representation of (C) frequency and (D) absolute number of ICOS+ Tregs on day 24. (E) Representative FACS plots demonstrating percentages of Foxp3+ CD4 and ICOS+ Tregs. (F) Tumor growth curves of B16F10 tumors in Foxp3DTR mice. Mice were first treated with anti-CTLA-4 (clone 9H10). Diphtheria toxin was administered to mice until the end of the experiment. Mice were then treated with CTLA-4 Ig (post) once the initial treatment of anti-CTLA-4 is finished. All experiments began with five mice per group. Representative experiment from duplicated experiments is shown.