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. 2024 May 22;5(6):101582. doi: 10.1016/j.xcrm.2024.101582

Figure 4.

Figure 4

DSRCT microenvironment displays immunosuppressive features and distinct CAF subpopulations

(A) Int_sc infiltrating myeloid (left panel) and lymphoid (right panel) subpopulation proportions represented by pie charts.

(B) UMAP plots displaying CAFs according to patient ID (left panel), tissue of origin (middle panel), and subclustering (right panel).

(C) Heatmap highlighting expression Z score of the top 50 DEGs of CAFs subcluster.

(D) Violin plot showing expression profile of CAF subclusters for canonical markers.

(E) Violin plots displaying expression profile of CAF subclusters for immunosuppressive markers.

(F) DSRCT bulk RNA-seq CAF subpopulations deconvolution.

(G) Immunofluorescence triplex showing ACTA2 (red), MCAM (yellow), FAP (green), and DAPI staining on a DSRCT FFPE sample in distinct stromal areas. The #1 annotation designates desmoplastic stromal areas, #2 shows tumor cell islets periphery, and #3 indicates the tumor pseudocapsule. The ∗ sign shows tumor necrosis. The scale bar is displayed on the bottom left corner of each panel, representing 1 mm on the left panel and 100 μm on the three right panels.