TABLE 1.

Transduction of dividing CaCo-2 and DGBE cells^a

Cells and vector	% GFP-positive cells	Mean fluorescence intensity
CaCo-2
Untransfected	2.5	23.1
pLNCeGFP	44	140.6
pRtatpeGFP	99	2,781
pRRLcmveGFPsin	76	287.6
DGBE
Untransfected	1	22.6
pLNCeGFP	89	2,229
pRtatpeGFP	87	2,498
pRRLcmveGFPsin	96	1,609

^aSubconfluent CaCo-2 and DGBE cells were infected with murine retrovirus LNCeGFP, first-generation lentivirus pRtatpeGFP, and third-generation lentivirus pRRLcmveGFPsin. Cells were analyzed by flow cytometry. All viral vectors transduced target cells efficiently. Mean fluorescence levels are similar in DGBE cells, and pRtatpeGFP mediates higher expression levels in CaCo-2 cells.