TABLE 2.
Cells and vector | % GFP-positive cellsb
|
|
---|---|---|
Apical infection | Basolateral infection | |
CaCo-2 | ||
pLNCeGFP | 2.6 ± 2 | <0.5 |
pRtatpeGFP | 43 ± 17 | 4.7 ± 1 |
pRRLcmveGFPsin | 24 ± 16 | <0.5 |
DGBE | ||
pLNCeGFP | 2.9 ± 1 | 2.4 ± 1 |
pRtatpeGFP | 33 ± 25 | <0.5 |
pRRLcmveGFPsin | 3.8 ± 0.5 | <0.5 |
Confluent monolayers of DGBE and CaCo-2 cells were transduced with first- and third-generation lentivirus vectors and a murine retroviral vector. Experiments were performed using at least two different viral preparations per virus type.
Results are averages and standard deviations from at least three different independent experiments.