Figure 1.
Comparison of mice after infection with low or high infection dose of influenza A virus. C57BL/6 mice were infected with either low dose (3 x 103 pfu, blue line) or high infection dose (3 x105 pfu, red line) of influenza A virus strain A/Regensburg/D6/09. (A) Scheme of the mouse survival study. Change of body weight, disease symptoms score, and survival were observed until day 20 p.i (n = 3). Figure created with BioRender.com. (B) Mean body weight change of infected mice (n = 3). Mice that lost over 25% of their initial body weight (grey dotted line) were sacrificed according to animal protection law. (C) Percentage of survival. Significant differences were analyzed by log-rank (Mantel-Cox) test (*p< 0.05). (D) Scheme of the mouse study for virus titer assessments. Figure was created with BioRender.com. (E) Virus titers of the lung are given as log10 in ffu per 1 ml organ-homogenate (n=3). Values are delineated as mean ± SD. The detection limit of this test was <1.7 log10 ffu/ml. Significance was analyzed by two-way ANOVA test (* p< 0.05). ns means not significant.