Figure 2.

Amount of CD4⁺ T cells, CD8⁺ T cells and Tregs in lung and thymus of mice infected with either low or high dose of influenza A virus. Mice were infected with either a low dose (blue bars) or high dose (red bars) of IAV. Uninfected mice were used as control (grey dashed line). Lungs and thymi were harvested and analyzed by flow cytometry for the presence of different T cell populations at day 3, 6 or 9 compared to uninfected control mice (grey dashed line). (A) CD4⁺ T cell, (C) CD8⁺ T cell and (E) Treg population in the lung and (B) CD4⁺ T cell, (D) CD8⁺ T cell and (F) Tregs in the thymus were analyzed. Data presented as mean ± SD (n = 3). Two-way ANOVA revealed significant differences between the two groups at the same time point (black * with brackets, p< 0.05).