TABLE 2.
List of strains used in this study.
| Strain | Host | Relevant genotype | Source |
|---|---|---|---|
| Escherichia coli DH5α | - | fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB |
| Escherichia coli BL21 (DE3) | - | F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Nzytech |
| Escherichia coli JM109 (DE3) | - | endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F′, traD36, proAB, lacIqZΔM15], lDE3 | Promega |
| TYR1 | Escherichia coli BL21 (DE3) | ΔpheLA ΔtyrR | This study |
| TYR2 | Escherichia coli JM109 (DE3) | ΔpheLA ΔtyrR | This study |
| LD1 | E. coli BL21 (DE3) | pETDuet_tyr | This study |
| LD2 | E. coli JM109 (DE3) | pETDuet_tyr | This study |
| LD3 | E. coli BL21 (DE3) | pETDuet_tyr* | This study |
| LD4 | E. coli JM109 (DE3) | pETDuet_tyr* | This study |
| LD5 | E. coli MG1655 | pETDuet_tyr | This study |
| LD6 | E. coli MG1655 | pETDuet_tyr* | This study |
| LD7 | E. coli BL21 (DE3) | pETDuet_Ptrc2_tyr* | This study |
| LD8 | E. coli JM109 (DE3) | pETDuet_Ptrc2_tyr* | This study |
| LD9 | TYR1 | pETDuet_tyr* | This study |
| LD10 | TYR2 | pETDuet_tyr* | This study |
| LD11 | TYR1 | pETDuet_Ptrc2_tyr* | This study |
| LD12 | TYR2 | pETDuet_Ptrc2_tyr* | This study |
| DPM1 | E. coli BL21 (DE3) | pETDuet_tyr_ddc | This study |
| DPM2 | E. coli JM109 (DE3) | pETDuet_tyr_ddc | This study |
| DPM3 | E. coli BL21 (DE3) | pETDuet_tyr*_ddc | This study |
| DPM4 | E. coli JM109 (DE3) | pETDuet_tyr*_ddc | This study |
| DPM5 | E. coli BL21 (DE3) | pETDuet_ddc | This study |
| DPM6 | E. coli JM109 (DE3) | pETDuet_ddc | This study |
| DPM7 | E. coli K12 MG1655 | pETDuet_tyr_ddc | This study |
| DPM8 | E. coli K12 MG1655 | pETDuet_tyr*_ddc | This study |
| DPM9 | E. coli BL21 (DE3) | pETDuet_Ptrcs_tyr_ddc | This study |
| DPM10 | E. coli JM109 (DE3) | pETDuet_Ptrcs_tyr_ddc | This study |
| DPM11 | E. coli BL21 (DE3) | pETDuet_Ptrcs_tyr*_ddc | This study |
| DPM12 | E. coli JM109 (DE3) | pETDuet_Ptrcs_tyr*_ddc | This study |
| DPM13 | TYR1 | pETDuet_tyr*_ddc | This study |
| DPM14 | TYR2 | pETDuet_tyr*_ddc | This study |
| DPM15 | TYR1 | pETDuet_Ptrcs_tyr*_ddc | This study |
| DPM16 | TYR2 | pETDuet_Ptrcs_tyr*_ddc | This study |
| DPA1 | E. coli BL21 (DE3) | pETDuet_tdc_ppoAB | This study |
| DPA2 | E. coli BL21 (DE3) | pETDuet_tdc_ppoMP | This study |
| DPA3 | E. coli JM109 (DE3) | pETDuet_tdc_ppoAB | This study |
| DPA4 | E. coli JM109 (DE3) | pETDuet_tdc_ppoMP | This study |
| DPA5 | E. coli BL21 (DE3) | pETDuet_Ptrcs_tdc_ppoAB | This study |
| DPA6 | E. coli BL21 (DE3) | pETDuet_Ptrcs_tdc_ppoMP | This study |
| DPA7 | E. coli JM109 (DE3) | pETDuet_Ptrcs_tdc_ppoAB | This study |
| DPA8 | E. coli JM109 (DE3) | pETDuet_Ptrcs_tdc_ppoMP | This study |
| DPA9 | E. coli BL21 (DE3) | pETDuet_tdc*_ppoMP | This study |
| DPA10 | E. coli JM109 (DE3) | pETDuet_tdc*_ppoMP | This study |
| DPA11 | E. coli BL21 (DE3) | pETDuet_Ptrcs_tdc*_ppoMP | This study |
| DPA12 | E. coli JM109 (DE3) | pETDuet_Ptrcs_tdc*_ppoMP | This study |
| DPA13 | TYR1 | pETDuet_tdc*_ppoMP | This study |
| DPA14 | TYR2 | pETDuet_tdc*_ppoMP | This study |
| DPA15 | TYR1 | pETDuet_Ptrcs_tdc*_ppoMP | This study |
| DPA16 | TYR2 | pETDuet_Ptrcs_tdc*_ppoMP | This study |
| DPA17 | E. coli BL21 (DE3) | pETDuet_tdc | This study |
| DPA18 | E. coli BL21 (DE3) | pETDuet_ppoMP | This study |