Figure 6.

Stages of membrane binding and ceramide release in the model system (Golgi bilayer). (A) Built initial complex of START, ceramide, and POPC. (B,C) Ceramide release to the bilayer. (D–F) Diacylglycerol lipid (POGL) uptake (and release) from (to) the bilayer. Bound ceramide molecule, colored yellow; POPC molecule, colored purple; residues in the binding site, colored in green. POGL is shown in magenta and bound in the cavity. Ω1 loop, colored orange; Ω4 loop, colored cyan; START domain, colored gray. The membrane is shown as a gray transparent surface.