FIG. 4.

Patient 1 (A) and patient 2 (B) CTL recognition of capsid components. CTLs were tested for 10 min at 37°C by an ELISPOT assay for the secretion of IFN-γ in the presence of autologous LCL (5,000 cells/well) loaded using a hypertonic solution (0.5 M sucrose, 10% polyethylene glycol 1,000, and 10 mM HEPES in RPMI 1640) with 20 μl of recombinant capsid components (penton base, hexon, and fiber) or control Sf9 cell extract (Sf9 plus 500 μl of hypertonic solution). Adenovirus-infected targets (12,000 particles of Ad-CFTR per cell) were used as positive controls, and the results are expressed as the number of specific spots per well after subtracting the background determined with uninfected unloaded LCL.