Figure 3.

A-MYB binds the Cyclin B2 promotor dependent on the cell cycle. RPE-1 cells were cultivated under growth factor withdrawal (starved) und restimulated by growth factor addition to distinguish between a G₀- and an S/G₂/M-enriched condition. Promoter binding of DREAM and MMB components was analyzed by ChIP. The relative enrichment of the Cyclin B2 promoter (A), the ORC1 promoter (early cell cycle gene) (D), and the negative control GAPDHS promoter (G) is visualized relative to the input DNA (representative experiment of n = 3). Binding of DREAM components to the Cyclin B2 promoter (B), the ORC1 promoter (E), and the GAPDHS promoter (H) was analyzed relative to the IgG negative control of the starved condition (n = 3). Binding of the activating MMB components B-MYB and FOXM1 as well as A-MYB to the Cyclin B2 promoter (C), the ORC1 promoter (F), and the GAPDHS promoter (I) was analyzed relative to the IgG negative control of the starved condition (n = 3).). Mean ± SD are given, and significances were calculated by two-way ANOVA (*P < 0.05; **P < 0.01; ***P < 0.001).